Laboratory Filter Plate

The AcroPrep 24-well Cell Clarification and Sterile Filtration Plate performs cell clarification and sterile filtration of CHO, HEK or other cultured cells in one step, in a single device, in minutes. This filter plate is ideal for laboratories that desire faster and more efficient protein purification workflows.

By combining both clarification and sterilization steps, the plate eliminates the need to harvest the cells in an offline centrifugation step, saving time. The filter plate's innovative one-step workflow uses multi-layer filtration media and membranes integrated into one device. The top layer features Pall's Seitz depth media that efficiently captures whole cells and cell debris. A 0.2 μm Supor EKV membrane provides an efficient sterile filtration layer. This combination effortlessly recovers more than 95% of extracellular proteins from whole-cell cultures of up to 25 million (or more) cells/mL of varying viabilities.

Additionally, this device can reduce plastic consumable use substantially.  In one example, it reduced plastic use by mass of seven times versus a conventional two-step process, significantly reducing disposal costs and environmental burden.

Pall also introduced a second AcroPrep 24-Well filter plate, the AcroPrep 24-well 0.2 µm Sterile Filtration Plate, for applications where a sterile filtrate is required. The device is designed for high-volume (up to 7 mL) processing of media, reagents, serum, or proteins. The 24-well Supor EKV plate features a dual-layer configuration, a 0.65 μm membrane integrated with a highly asymmetric 0.2 μm membrane for fast, efficient, and sterilizing-grade filtration. The gamma-irradiated device demonstrates retention of a minimum of 107 cfu/cm2 of Brevundimonas diminuta per modified ASTM F838.

Both AcroPrep 24-well filter plates can be used with either a vacuum manifold or compatible centrifuge and are fully compatible with all major laboratory automation platforms.  Each filter plate comes individually bagged with a collection plate and lid.  The devices are ideal for clone selection and clone candidate analysis, cell expansion studies, recombinant protein isolation prior to analysis, cell clarification, process optimization, and general sterile filtration.